IgG3, Mouse, BioAssay(TM) ELISA Kit

Immunoassays using heavy-chain specific antibodies provide for selective, sensitive quantification of mouse immunoglobulins IgG (and subclasses), IgA and IgM, as found circulating in blood or as present in other body fluids, including saliva, milk/colostrum, ascites, tears and mucosa of linings of the gut, respiratory or urigenital tracts.\n\nLevels of total IgG (and subclasses), IgA and/or IgM can reveal health status or results of experimental or pathological conditions (e.g., hypo- or hypergammaglobulinemia or acute or chronic infection). Also, measurements of specific antibody levels, in antigen-specific assays, are often best interpreted relative to values of total IgG, IgA, and IgM in the sample and/or individual.\n\nThe assays are also suitable for quantifying monoclonal antibodies and can be a reliable method for monitoring production and establishing quality control.\n\nThe quantitative immunoassays measure mouse IgG and subclasses, IgA and IgM with high sensitivity; this allows dilution beyond interference from the sample matrix for samples derived from any of the above specimen types. Expected performance of each kit relative to precision, recovery and linearity of dilution is presented for guidance of use and experimental design.\n\nSample Type:\nSerum. Plasma, Culture medium and other fluids may be adapted.\n\nSensitivity:\n25ng/ml; 105 min\n\nRange:\nAssay of IgG3 in stored sera from twenty (20) individual Swiss mice ranged from 1.4-6mg/ml (median = 3.4mg/ml). Each laboratory should determine expected values of its own testing population.\n\nIntended Use:\nThe Mouse IgG3 ELISA Kit is an in vitro immunoassay for the quantification of IgG3 circulating in serum or in other appropriately qualified samples from tissue fluids (e.g., saliva, mucosa), or in cultures of mouse cells.\n\nTest Principle:\nThe Mouse IgG3 ELISA kit is based on the binding of mouse IgG3 in samples to two antibodies, one immobilized on the microtiter wells, and the other conjugated to horseradish peroxidase (HRP) enzyme. After a washing step, chromogenic substrate is added and color is developed by the enzymatic reaction of HRP on the TMB substrate, which is directly proportional to the amount of IgG3 present in the sample. Stopping Solution is added to terminate the reaction, and absorbance at 450nm is then measured using an ELISA microtiter well reader. The concentration of IgG3 in samples and control is calculated from a curve of standards containing known concentrations of IgG3.\n\nKit Components:\nAnti-Mouse IgG3 Microwell Strip Plate: 12X8-well strips\nMouse IgG3 Control: 1X0.65ml\nMouse IgG3 Standard (50ng/ml): 1X0.65ml\nMouse IgG3 Standard (100ng/ml): 1X0.65ml\nMouse IgG3 Standard (400ng/ml): 1X0.65ml\nMouse IgG3 Standard (400ng/ml): 1X0.65ml\nMouse IgG3 Standard (1000ng/ml): 1X0.65ml\nAnti-Mouse IgG3 HRP Conjugate (100X): 1X0.15ml\nSample Diluent Concentrate (20X): 1X10ml\nWash Solution Concentrate (100X): 1X10ml\nTMB Substrate: 1X12ml\nStop Solution: 1X12ml\n\nStorage and Stability:\nStore liquids at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

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