IkB(alpha) (Phospho-Ser32/36) Monoclonal Antibody (Clone 39A1413)

Antigen: synthetic peptide containing phosphorylated serine residues corresponding to human IkB(alpha) amino acids 32 and 36 . Host: mouse, clone 39A1413 . Isotype: IgG1k . Cross Reactivity: (+) bovine, canine, human, mouse, porcine, and rat IkB(alpha) . Application(s): WB and IP . External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to activation of the IkB-kinase complex that specifically phosphorylates IkB(alpha) on serine32 and serine36. Phosphorylation of these sites leads to ubiquitination of IkB(alpha) and subsequent degradation by the 26S proteasome. Degradation of IkB(alpha) results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocate to the nucleus and activate target genes.

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SPECIFICATIONS

Catalog Number

13923

Applications

IP, WB

Hosts

Mouse

Form

1 ea

P Type

Antibodies|NF-.kappa.B|Intracellular Signaling

Weight

0

Antigen

synthetic peptide containing phosphorylated serine residues corresponding to human IkB(alpha) amino acids 32 and 36

Isotype

IgG1k

Storage Temp

-20

Shipping Temp

-20

Additional Info

NF-kB is silenced in the cytoplasm by an inhibitory protein IkB. Synthesis of IkB(alpha) is autoregulated. IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/(alpha), IKK2/(beta), and IKK3/(gamma). External stimuli such as tumor necrosis factor or other cytokines initiates a signal transduction cascade that leads to activation of the IkB-kinase complex that specifically phosphorylates IkBa on serine32 and serine36. Phosphorylation of these sites leads to ubiquitination of IkB(alpha) and subsequent degradation by the 26S proteasome. Degradation of IkB(alpha) results in unmasking of the nuclear localization signal of NF-kB dimers, which subsequently translocate to the nucleus and activate target genes. Six members of the IkB family members have been identified. one of the first genes induced following NF-kB activation is IkB(alpha).