IKK epsilon, phosphorylated (Thr501) (IKKe)

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb and\nIKKgg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has\nsignificant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution: \nELISA: 1:2500-1:5000\nWestern Blot: 1:500\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nHeLa cells or TNF inducible KBM-5 cells\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I3000-62

Size

100ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%. No stabilizing proteins added.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Peters, R.t Liao, S.M. and Maniatis, T. (2000) IKKepsilon is part of a novel PMA-inducible IkappaB kinase complex. Mol Cell 5;(3):513-522. 2. Shimada, T. et al (1999) IKK-i, a novel lipopolysaccharide-inducible kinase that is related to IkappaB kinases. Int. Immunol 11(8): 1357-1362.

Additional Info

Recognizes human IKKe pT501, phosphorylated, Mr 85kD. Shows minimal reactivity by Western Blot with non-phosphorylated IKKe.