IL-12(p40) BioAssay(TM) ELISA Kit, Human

Sandwich ELISA quantitative detection kit for Human IL-12(p40) in cell culture supernates, serum and plasma (heparin, EDTA, citrate).\n\nInterleukin-12 (IL12; formerly NKSF, for natural killer cell stimulatory factor, or CLMF, for cytotoxic lymphocyte maturation factor) is a novel cytokine cloned from B-cell lines. IL-12 is a heterodimeric molecule composed of p35 and p40 subunits.1 The larger 40kD subunit (p40) is a member of the cytokine receptor family, and the smaller 35kD subunit (p35) is related to IL6 and GCSF.2 Both IL-12 p40(-/-) and p35(-/-) mice fail to produce IL-12 p70 heterodimer.3 Interleukin (IL)-12 has been cloned on the basis of its ability to activate natural killer (NK) cells and promote the development of cytolytic T cells. With further understanding of its activities, IL-12 has emerged as an important cytokine, affecting both immune and hematologic functions. It has been shown to be necessary for the T cell independent induction of interferon (IFN)-gamma, critical for the initial suppression of bacterial and parasitic infection; for the development of a Th1 response, critical for effective host defense against intracellular pathogens; and for the activation of differentiated T lymphocytes of both CD4+ and CD8+ phenotype.4 The subunits map to different chromosomes: p40 (IL12B) to 5q31-q33 and p35 (IL12A) to 3p12-3q13.2.2 The standard product used in this kit is recombinant human IL-12(p40), which is a 40kD glycoprotein consisting of 306 amino acids.\n\nAssay Range:\n31.2pg/ml-2000pg/ml\n\nSensitivity:\n< 2 pg/ml\n\nCrossreactivity:\nThere is cross-reactivates with IL-12(p70), IL-23(p19/p40) < 5%.\n\nUniprot ID:\nP29460\n\nEntrez Gene ID:\n3593\n\nKit Components:\n96-well plate pre-coated with anti-IL-12(p40), Human\nLyophilized recombinant IL-12(p40), Human standard, 10ng/tube, 2Ea\nUSB Cat No Kit Component\n143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution)\n143697 Sample diluent buffer, 30ml\n143698 Antibody diluent buffer, 12ml\n143699 ABC diluent buffer, 12ml\n143700 TMB color developing agent, 10ml\n143701 TMB stop solution, 10ml\n\nStorage and Handling:\nStore at 4 degrees C for 6 months, at -20 degrees C for 12 months. Avoid multiple freeze-thaw cycles.

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SPECIFICATIONS

Catalog Number

143891

Size

96Tests

References

1. Cua, D. J.; Sherlock, J.; Chen, Y.; Murphy, C. A.; Joyce, B.; Seymour, B.; Lucian, L.; To, W.; Kwan, S.; Churakova, T.; Zurawski, S.; Wiekowski, M.; Lira, S. A.; Gorman, D.; Kastelein, R. A.; Sedgwick, J. D. Interleukin-23 rather than interleukin-12 is the critical cytokine for autoimmune inflammation of the brain. Nature 421: 744-748, 2003. \n2. Sieburth, D.; Jabs, E. W.; Warrington, J. A.; Li, X.; Lasota, J.; LaForgia, S.; Kelleher, K.; Huebner, K.; Wasmuth, J. J.; Wolf, S. F. Assignment of genes encoding a unique cytokine (IL12) composed of two unrelated subunits to chromosomes 3 and 5. Genomics 14: 59-62, 1992. \n3. Becher, B.; Durrell, B. G.; Noelle, R. J. Experimental autoimmune encephalitis and inflammation in the absence of interleukin-12. J. Clin. Invest. 110: 493-497, 2002.\n4. Wolf, S. F.; Sieburth, D.; Sypek, J. Interleukin 12: a key modulator of immune function. Stem Cells 12: 154-168, 1994. \n