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Immunohistochemistry Detection Kit, HRP (DAB, Broad Spectrum) BioAssay(TM)

Cat no: I7506-07

Immunohistochemistry Detection Kit, HRP (DAB, Broad Spectrum) BioAssay(TM)

This Immunohistochemistry Detection Kit, HRP (DAB, Broad Spectrum), BioAssay(TM) is a proprietary and enhanced HRP polymer technology designed to provide excellent sensitivity, high specificity, and a shorter protocol than conventional immunohistochemical staining methods. New polymer conjugation methods have resulted in a versatile polymer capable of intense nuclear, cytoplasmic, and membrane antigen staining. The polymer conjugate contains multiple HRPs to provide excellent sensitivity and specificity. The polymer staining method is faster and easier than a conventional LAB-SA protocol because it uses an HRP polymer conjugated to the second antibody and eliminates one incubation step. In addition, the kit does not contain biotin nor streptavidin, so potential background due to endogenous biotin activity is completely avoided. Another advantage is the shorter polymer incubation time; the system only requires 10 mintues, while leading competitors polymers require 30 minutes.\n\nReactivity: \nMouse, rabbit, rat, guinea pig\n\nApplications: \nSuitable for use in Immunohistochemistry (FFPE). Other applications not tested.\n\nKit Components:\nI7506-07A: One dropper bottle (15ml) of ready-to-use HRP polymer conjugate.\nI7506-07B: One dropper bottle (3ml) of 20X buffer/substrate\nI7506-07C: One dropper bottle (3ml) of 20X DAB chromogen\nI7506-07D: One dropper bottle (3ml) of 20X 0.6% H2O2\n\nReagents Required But Not Supplied:\n

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SPECIFICATIONS

Catalog Number

I7506-07

Size

150 Slides

Applications

IHC

Reactivities

Mouse, Rat, GP, Rabbit

References

1. Petrosyan, K., et al., J. Histotechnology 25(4): 247-250 (2002).

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