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iNOS Polyclonal Antibody

Cat no: 160862

iNOS Polyclonal Antibody

Antigen: purified enzyme from mouse macrophages (RAW 264.7 cells) . Host: rabbit . Cross Reactivity: (+) iNOS from most mammalian species and nNOS (~5%); (-) eNOS . Application(s): ICC, IP, and WB . NOS catalyzes the biosynthesis of nitric oxide from L-arginine. iNOS is a soluble enzyme found in a variety of tissues including macrophages, hepatocytes, vascular smooth muscle cells, and chondrocytes. iNOS expression is increased by a variety of factors including LPS, IFN-g, IL-1b, and TNF-a.

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SPECIFICATIONS

Catalog Number

160862

Applications

ICC, IP, WB

Hosts

Rabbit

Form

1 ea

P Type

Antibodies

Weight

0

Antigen

purified enzyme from mouse macrophages (RAW 264.7) cells

Storage Temp

-20

Shipping Temp

-20

Additional Info

Nitric Oxide Synthase (NOS) catalyzes the biosynthesis of nitric oxide from L-arginine. Constitutively expressed NOS is found in brain (nNOS) and endothelial cells (eNOS). iNOS is a soluble enzyme found in a variety of tissues including macrophages, hepatocytes, vascular smooth muscle cells, and chondrocytes. iNOS expression is increased by a variety of factors including LPS, IFN-.gamma., Il-1.beta., and TNF-.alpha., whereas expression is deceased by dexamethasone. The enzyme has been cloned from several species including mouse, rat, and human with homology of at least 80% between these species. The calculated molecular weight of the protein from the deduced amino acid sequence is 130,000 - 131,000.

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