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Inositol Polyphosphate 4-phosphatase Type II (Inositol Polyphosphate-4-phosphatase Type II 105kD, INPP4B, MGC132014)

Cat no: I7655-55

Inositol Polyphosphate 4-phosphatase Type II (Inositol Polyphosphate-4-phosphatase Type II 105kD, INPP4B, MGC132014)

Inositol polyphosphate 4-phosphatase (INPP4B) is an Mg2+ independent enzyme involved in phosphatidylinositol signaling pathways. INPP4B type II catalyses the hydrolysis of the 4-position phosphate from phosphatidylinositol 3,4-bisphosphate. 1,3,4-trisphosphate, and inositol 3,4-bisphosphate. This process rapidly generates two second messengers phosphatidylinositol 3,4-bisphosphate and phosphatidylinositol 3,4,5-trisphosphate in response to extracellular signal (1). INPP4B and its secondary messengers have been linked to cell growth, differentiation, apoptosis, protein trafficking, and cell motility (2). It is suggested that INPP4B type II might be subject to alternative splicing, similar to type I enzyme. However, additional factors and/or processing maybe required for alternative isozymes to be active (3).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Flow Cytometry. Not recommended for use in Immunofluorescence or Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000-1:5000 detects a band at ~107kD\nImmunohistochemistry (paraffin): 1:50\nFlow Cytometry: 1:50\nOptimal dilutions to be determined by the researcher.\n\nPositive Control: \nMCF7, fetal skeletal muscle, fetal heart lysates and muscle tissue\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.\n\nManufactured incorporating RabMAb(R) technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.

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SPECIFICATIONS

Catalog Number

I7655-55

Size

100ul

Applications

FC, IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a liquid in PBS, pH 7.4, 0.05% BSA, 0.01% sodium azide, 50% glycerol.

P Type

Mab

Purity

Supernatant

Isotype

IgG

References

1. Norris F et al. JBC, 272(17):10987-10989, 1997. 2. Ferron M, Vacher J. Gene, 376(1):152-61. 2006.

Additional Info

Recognizes human Inositol Polyphosphate 4-phosphatase Type II. Species Crossreactivity: mouse, rat.

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