INPPL1 (Inositol polyphosphate phosphatase-like protein 1)

INPPL1 (Inositol polyphosphate phosphatase-like 1), also called SHIP2, encodes inositol polyphosphate-5 phosphatase-like 1, a protein that in addition to the phosphatase domain contains an SH2 (src-homology domain 2) motif. INPPL1 is a member of the family of inositol triphosphate phosphatases. The INPPL1 gene is mapped on 11q13.4. Immunoblot analysis revealed that SHIP2 was widely expressed in fibroblast and nonhematopoietic tumor cell lines, unlike the SHIP protein, which was found only in cell lines of hematopoietic origin. Using human epithelial cell lines and primary human corneal and epidermal keratinocytes, Yu et al. (2008) showed that microRNA-184 (MIR184) interfered with the ability of MIR205 to downregulate expression of SHIP2.SHIP2 may play a significant role in regulation of phosphatidylinositol 3-prime-kinase signaling by growth factors and insulin. By targeting the translation-initiating ATG codon and deleting the first 18 exons encoding Inppl1, Sleeman et al. (2005) generated Inppl1 -/- mice that were null for Inppl1 mRNA and protein.\n\nUniProt Number:\nO15357\n\nGene ID:\nINPPL1\n\nApplications:\nSuitable for use in Western Blot and Immunohistochemistry (Paraffin).\n\nRecommended Dilution:\nOptimal dilutions to be determined by the researcher.\n\nStorage and Handling:\nStore at -20?C for one year. After reconstitution, store at 4?C for one month. Can also be aliquoted and stored frozen at -20?C for long term.\nAvoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

144757

Size

100ug

Applications

IHC, WB

Hosts

Rabbit

Reactivities

Hum, Mouse, Rat

Form

Supplied as a lyophilized powder. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3. Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1.Habib, T., Hejna, J. A., Moses, R. E., Decker, S. J. Growth factors and insulin stimulate tyrosine phosphorylation of the 51C/SHIP2 protein. J. Biol. Chem. 273: 18605-18609, 1998.\n2.Sleeman, M. W., Wortley, K. E., Lai, K.-M. V., Gowen, L. C., Kintner, J., Kline, W. O., Garcia, K., Stitt, T. N., Yancopoulos, G. D., Wiegand, S. J., Glass, D. J. Absence of the lipid phosphatase SHIP2 confers resistance to dietary obesity. Nature Med. 11: 199-205, 2005.\n3.Yu, J., Ryan, D. G., Getsios, S., Oliveira-Fernandes, M., Fatima, A., Lavker, R. M. MicroRNA-184 antagonizes microRNA-205 to maintain SHIP2 levels in epithelia. Proc. Nat. Acad. Sci. 105: 19300-19305, 2008.\n

Additional Info

Recognizes human, mouse and rat INPPL1. No crossreactivity with other proteins.