Insulin-Like Growth Factor 1 Receptor, Recombinant, Human (IGF1R)

IGF-1R (Insulin-like Growth Factor-1 Receptor) consists of alpha- and beta-subunits which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The alpha-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. This recombinant human IGF-IR protein encodes amino acids 1213-1367.\n\nProtein Family: \nBelongs to the Tyr protein kinase family. Insulin\n\nApplications: \nSuitable for use in Western Blot, ELISA and Inhibition Assays. Other applications not tested.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7661-20H

Size

2ug

Form

Supplied as a liquid in 50mM Tris-acetate, pH 7.5, 1mM EDTA and 20% glycerol.

Purity

On SDS-PAGE commassie blue stained gel, the purified recombinant protein shows a single band at 43kD (including GST tag).

References

1. Hydrogen peroxide inhibits activity of the IGF-1 receptor kinase. Redox Rep (2006) 11:105-109. 2. Model for the complex between the insulin-like growth factor I and its receptor: towards designing antagonists for the IGF-1 receptor. Protein Eng Des Sel (2006) 13; 3. Blockade of IGF-1 receptor tyrosine kinase has antineoplastic effects in hepatocellular carcinoma cells. Biochem Pharmacol (2006) 71:1435-1448. 4. Neuroprotective effects of activated protein C through induction of insulin-like growth factor-1 (IGF-1), IGF-1 receptor, and its downstream signal phosphorylated serine-threonine kinase after spinal cord ischemia in rabbits. Stroke (2006) 37:1081-1086. 5. IGF-1 receptor tyrosine kinase inhibition by the cyclolignan PPP induces G2/M-phase accumulation and apoptosis in multiple myeloma cells. Blood (2006) 107:669-678. 6. Inhibiting the IGF-1 receptor tyrosine kinase with the cyclolignan PPP: an in vitro and in vivo study in the 5T33MM mouse model. Blood (2006) 107:655-660.