Insulin-Like Growth Factor Binding Protein 1 (IGFBP-L1) (Biotin)

Insulin-like Growth Factor Binding Protein-Like 1 (also IGFBP-rP4) is a secreted member of the IGFBP family of proteins. Based on human, mature mouse IGFBP-L1 is a 251 amino acid (aa), 38kD glycoprotein. It contains an N-terminal IGFBP motif (aa 53-68), a kazal-type serine protease inhibitor region (aa 83-143) and a C-terminal Ig-like domain (aa 161-237). IGFBP-L1 is expressed in brain and testis and may be a tumor suppressor, possibly through the induction of apoptosis. Over aa 18-270, mouse IGFBP-L1 shares 96% and 79% sequence identity with rat and human IGFBP-L1, respectively.\n\nApplications: \nSuitable for use in Western Blot and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 0.1-0.2ug/mL detection limit for rmIGFBP-L1 ~ 2ng/lane and 5ng/lane under non-reducing and reducing conditions, respectively.\nImmunohistochemistry: 10ug/mL detects IGFBP-L1 in cryosections of E13.5 mouse brain. Tissue was fixed overnight in 4% PFA plus14% picric acid in PBS at 4 degrees C. Sections were blocked with 10% donkey serum and 0.1% Triton in 1% BSA in PBS for 45 minutes at RT. After blocking, sections were incubated with diluted primary antibody for 3 hours at RT followed by dye-conjugated streptavidin at RT for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-16B

Size

50ug

Applications

IHC, WB

Hosts

Goat

Reactivities

Mouse

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS containing 50ug of bovine serum albumin (BSA) per 1ug of antibody. Labeled with Biotin. Reconstitute with sterile Tris-buffered saline, pH 7.3 (20 mM Trizma base, 150 mM NaCl) containing 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes mouse IGFBP-L1.