Insulin-Like Growth Factor Binding Protein 2 (IGFBP2, BP2, IBP2)

Insulin-like growth factor-binding proteins (IGFBPs) play an integral role in modifying insulin-like growth factor (IGF) actions in a wide variety of cell types. This family contains six members that are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, others potentiate IGF actions by facilitating the ligand-receptor interaction (1-3). IGFBP-2 is the second most abundant IGFBP in the circulation and is present in various other biological fluids and tissues of many vertebrate species. Serum IGFBP-2 levels are elevated in many acute or chronic nonphysiological situations, such as shock, fasting, hypoxemia or after traumata, suggesting complex regulation of IGFBP-2 expression (4). IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis (5).\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:1000\nImmunohistochemistry (Paraffin) 1:25\nImmunoprecipitation: 1:50\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7661-16E

Size

100ul

Applications

IHC, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and peptide affinity chromatography.

Isotype

IgG

References

1. Duan, C., J. Endocrinol. 175: 41-54 (2002). 2. Sandhu, M.S., et al., J. Natl. Cancer Inst. 94: 972-980 (2002). 3. Baxter, R.C., Best Pract. Res. Clin. Endocrinol. Metab. 15: 421-434 (2001). 4. Noel, M., et al., Curr. Opin. Clin. Nutr. Metab. Care 4: 399-405 (2001). 5. Zumkeller, W., Mol. Pathol. 54: 285-288 (2001).

Additional Info

Recognizes endogenous levels of total human IGFBP-2 protein at ~35kD. Does not crossreact with any other IGFBP family members.