Insulin-Like Growth Factor I, Recombinant, Human (IGF1, IGF I)

Insulin Like Growth Factor-I (IGF-I) is a polypeptide growth factor which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. Recombinant Human IGF-I is a single, nonglycosylated, 7.6kD protein containing 70 amino acid residues. Insulin-like growth factor 1 (IGF-I) is involved in regulation of neuronal growth and development in central and peripheral nervous system. It is known to protect neurons against cell death induced by amyloidogenic derivatives, glucose or serum\ndeprivation through pathways involving AKT kinase and transcription factor FKHRL1 phosphorylation. Activation of the insulin-like growth factor system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like breast and thyroid cancers.\n\nRecombinant protein corresponding to human expressed in E. coli\n\nAmino Acid Sequence:\nGPRTLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMY CAPLKPAKSA\n\nBiological Activity: \nEC50 is typically 21.6-54.7ng/ml. Measured using dose dependent stimuation of Balbc 3T3 cell line. \n\nStorage and Stabiltiy: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months after receipt at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-11D

Size

1mg

Form

Supplied as a lyophilized powder with no additives. Reconsitute in sterile ddH2O to a concentration of 0.1-1mg/ml. Also available as a liquid. See I7661-11C.

Purity

~98% by SDS-PAGE and HPLC analyses. Endotoxin:\n(same/less than)0.1ng/ug.