Insulin-Like Growth Factor I, Recombinant, Mouse (Insulin-like Growth Factor 1, Igf1, Igf-1, IGF-I, C730016P09Rik, Somatomedin)

Source:\nRecombinant corresponding to 70aa of human Insulin Like Growth Factor-1 expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~7.6kD\n\nBiological Activity:\nThe ED50, calculated by the dose-dependent proliferation of mouse BALBC 3T3 cells (measured by 3H-thymidine uptake) is less then 1.0ng/ml, corresponding to a specific activity of 1x10U/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 0.47 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences(IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IGF-I as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-10L

Size

50ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Organ-specific and age-dependent expression of insulin-like growth factor-I (IGF-I) mRNA variants: IGF-IA and IB mRNAs in the mouse. Zoolog Sci 2005 Sep;22(9):1011-21. 2. The impairment of IGF-I-stimulated protein synthesis and activation of protein kinase B, p70(S6k), MAP kinase, and p90(rsk) in mouse C2C12 myogenic cells exposed to high glucose and high insulin. Pol J Vet Sci 2005;8(3):241-50. 3. Studies involving the GH-IGF axis: Lessons from IGF-I and IGF-I receptor gene targeting mouse models. J Endocrinol Invest 2005;28(5 Suppl):19-22. 4. Methionine-deficient diet extends mouse lifespan, slows immune and lens aging, alters glucose, T4, IGF-I and insulin levels, and increases hepatocyte MIF levels and stress resistance. Aging Cell 2005 Jun;4(3):119-25. 5. Insulin-like growth factor-I stimulated DNA replication in mouse endometrial stromal cells. J Reprod Dev 2005 Jun;51(3):305-13. 6. Insulin-like growth factor-I stimulates cell proliferation in the outer layer of Hertwig's epithelial root sheath and elongation of the tooth root in mouse molars in vitro. Cell Tissue Res 2005 Apr;320(1):69-75.