Insulin-Like Growth Factor I, Recombinant, Rat (IGF1)

A well-characterized basic peptide believed to be secreted by the liver and to circulate in the blood. It has growth-regulating, insulin-like, and mitogenic activities. This growth factor has a major, but not absolute, dependence on Somatotropin. It is believed to be mainly active in adults in contrast to Insulin like Growth Factor 2, which is a major fetal growth factor. \n\nRecombinant Rat IGF-1 is a single, non-glycosylated, polypeptide chain containing 70 amino acids and having a molecular mass of 7687 Dalton. \n\nBiological Activity:\nRat IGF-1 is fully biologically active when compared to standards. The ED50, was calculated using 2 methods: \n1. Stimulation of protein synthesis in rat L6 myoblasts ED50 was found to be less then 30ng/ml. \n2. Type 1 IGF receptor binding assay ED50 was found to be less then 10ng/ml.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% HSA or BSA and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-10F

Size

10ug

Form

Supplied as a lyophilized powder in 100mM acetic acid. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to a concentration of > 0.1mg/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than) 95% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.

References

US Biological application reference: Kloting, N. et al., (2008) Diabetes 57:2074-2082. 1. Shimatzu, A. & Rotwein, P., J. Biol. Chem. 262: 7894-7900 (1987). 2. Upton, Z., et al., J. Endocrinol. 149: 379-387 (1996).