Insulin Receptor, active, Recombinant, Human

Recombinant human Insulin receptor residues 1005-1310, containing an N-terminal His6-tag. Expressed by baculovirus in Sf21 insect cells. \n\nInsulin Receptor Sequence Information Protein Human, Insulin R \nAccession number GenBank NM_000208 Tags N-terminal His6 Native sequence V1005 of native protein is equivalent to V16 in recombinant sequence. \n\nSpecific Activity: As reported\n\nUnit Definition: \nOne unit equals the incorporation of 1nmol of phosphate per min into 250uM Axltide (KKSRGDYMTMQIG), at 30 degrees C with a final ATP concentration of 100uM.\n\nApplications:\nSuitable for us ein Protein Phosphorylation Assays and MS. Tryptic Fingerprint. Other applications not tested.\n\nRecommended dilution:\nProtein Phosphorylation Assay: 30-300ng. Optimal dilutions to be determined by the researcher.\n\nStorage and Stability: \nAliquot to avoid repeated freezing and thawing and freeze at -70 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.\n\nRecombinant IR amino acid sequence: 1 MAHHHHHHEN LYFQGVFPCS VYVPDEWEVS REKITLLREL GQGSFGMVYE GNARDIIKGE 61 AETRVAVKTV NESASLRERI EFLNEASVMK GFTCHHVVRL LGVVSKGQPT LVVMELMAHG 121 DLKSYLRSLR PEAENNPGRP PPTLQEMIQM AAEIADGMAY LNAKKFVHRD LAARNCMVAH 181 DFTVKIGDFG MTRDIYETDY YRKGGKGLLP VRWMAPESLK DGVFTTSSDM WSFGVVLWEI 241 TSLAEQPYQG LSNEQVLKFV MDGGYLDQPD NCPERVTDLM RMCWQFNPKM RPTFLEIVNL 301 LKDDLHPSFP EVSFFHSEEN K \n\nRecombinant IR nucleotide sequence: 1 atggcgcatc accatcacca tcatgaaaac ctgtattttc agggcgtgtt tccatgctct 61 gtgtacgtgc cggacgagtg ggaggtgtct cgagagaaga tcaccctcct tcgagagctg 121 gggcagggct ccttcggcat ggtgtatgag ggcaatgcca gggacatcat caagggtgag 181 gcagagaccc gcgtggcggt gaagacggtc aacgagtcag ccagtctccg agagcggatt 241 gagttcctca atgaggcctc ggtcatgaag ggcttcacct gccatcacgt ggtgcgcctc 301 ctgggagtgg tgtccaaggg ccagcccacg ctggtggtga tggagctgat ggctcacgga 361 gacctgaaga gctacctccg ttctctgcgg ccagaggctg agaataatcc tggccgccct 421 ccccctaccc ttcaagagat gattcagatg gcggcagaga ttgctgacgg gatggcctac 481 ctgaacgcca agaagtttgt gcatcgggac ctggcagcga gaaactgcat ggtcgcccat 541 gattttactg tcaaaattgg agactttgga atgaccagag acatctatga aacggattac 601 taccggaaag ggggcaaggg tctgctccct gtacggtgga tggcaccgga gtccctgaag 661 gatggggtct tcaccacttc ttctgacatg tggtcctttg gcgtggtcct ttgggaaatc 721 accagcttgg cagaacagcc ttaccaaggc ctgtctaatg aacaggtgtt gaaatttgtc 781 atggatggag ggtatctgga tcaacccgac aactgtccag agagagtcac tgacctcatg 841 cgcatgtgct ggcaattcaa ccccaagatg aggccaacct tcctggagat tgtcaacctg 901 ctcaaggacg acctgcaccc cagctttcca gaggtgtcgt tcttccacag cgaggagaac 961 aagtaa\n\nStorage and Shelf Life: Stable for 1 year at-70 degrees C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated thawing and freezing.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

I7661-22G

Size

5ug

Form

Supplied as a frozen solution in 50mM Tris-HCl, pH 7.5, 0.1mM EGTA, 150mM sodium chloride, 1mM benzamidine, 0.03% Brij-35, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 270mM sucrose.

Purity

~80%. Purified using Ni-NTA agarose