Integrin alphaV, beta3

The integrin family of cell adhesion receptors consists of a least 16 membrane-associated heterodimers, composed of an alpha and beta subunit that associate in a non-covalent manner. The structure and functional diversity of the integrin family are based on the pairing abilities of the alpha and beta subunits. The integrins are involved in vascular proliferation, adhesion and wound repair. AlphaVbeta3 appears to be selectively expressed on growing blood vessels and has been identified as a marker of angiogenic vascular tissue.\n\nApplications: \nSuitable for use in Immunoprecipitation, Immunofluorescence, Flow Cytometry, Immunohistochemistry (frozen sections only), Immunocytochemistry and Blocking. Other applications have not been tested.\n\nRecommended Dilution:\nImmunoprecipitation: Precipitates integrin alphaV beta3 complex. When resolved by SDS-PAGE under reducing conditions, bands are observed at ~130kD and ~105kD corresponding to the alphaV and beta3 subunits, respectively.\nImmunofluorescence: 5-10ug/ml for staining of bFGF-treated 6um cryosections of chick chorioallantoic membrane fixed with acetone. Also effective on 4% paraformaldehyde fixed, frozen tissues and cells. Not effective for immunohistochemical staining of paraffin-embedded tissue sections. \nFlow Cytometry: 1-3ug/0.5x10e6 cells in 100ul volume\nBlocking: Inhibits adhesion of cells to vitronectin-coated surfaces at 10-25ug/ml \nOptimal dilutions to be determined by the researcher.\n\nRecommended Positive Control: \nHuman myeloma cell lines M21 and MoalphaV\n\nRecommended Negative Control: \nMo cells (this cell line was derived from M21 but does not express integrin alphaV)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

I7661-37E

Size

100ug

Applications

FC, ICC, IF, IHC, IP

Hosts

Mouse

Reactivities

Hum, Bov, Can, Ch/Bird, NHP, Prc, Rabbit

Form

Supplied as a liquid in PBS, pH 7.6. No preservative added.

P Type

Mab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG1

References

1. Ria, R., et al., Haematologica 87: 836-845 (2002). 2. Tsopanoglou, N.E., et al., Am. J. Physiol. Cell Physiol. 283: C1501-C1510 (2002). 3. Ratnikov, B.I., et al., J. Biol. Chem. 277: 7377-7385 (2002). 4. Gonzalez, A.M., et al., PNAS USA 99: 16,075-16,080 (2002). 5. Gao, B., et al., Amer. J. Physiol. Cell Physiol. 283: C1196-C1205 (2002). 6. Ahmed, N., et al., J. Cellular Biochemistry 84: 675-686 (2002). 7. Ahmed, N., et al., Carcinogenesis 23: 237-244 (2002). 8. Furumtsu, T., et al., J. Biochem. 131: 619-626 (2002).

Additional Info

Recognizes the human vitronectin receptor aVb3 complex, an RGD-directed adhesion receptor. Species Crossreactivity: Avian, bovine, canine, chicken, monkey, porcine and rabbit. Not reactive with rat.

Alternative Names

Vitronectin Receptor