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Integrin, beta5 (Biotin)

Cat no: I7661-43F

Integrin, beta5 (Biotin)

Integrin b5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin aV. Integrin aVb5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin b5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin b5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin b5, respectively.\n\nApplications: \nWestern Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Integrin b5. The detection limit for rhIntegrin b5 is approximately 2 ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 5% cross-reactivity with Integrin b1, -b2, -b3, rmIntegrin b4, -b6, and -b7. \nOptimal dilutions should be determined by each laboratory for each application.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-43F

Size

50ug

Applications

WB

Hosts

Sheep

Reactivities

Hum, Mouse, Can

Conjugates

Biotin

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is used, the concentration will be 50ug/ml.

P Type

Pab

Purity

Purified by human Integrin b5 affinity chromatography and then biotinylated.

Isotype

IgG

Additional Info

Selected for use as a detection antibody in human Integrin b5 Western Blots.

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