Integrin, beta5

Integrins are a/b heterodimeric cell surface receptors that play a pivotal role in cell adhesion and migration, as well as in growth and survival (1,2). The integrin family contains at least 18 a and 8 b subunits that form 24 known integrins having distinct tissue distribution and overlapping ligand specificities (3). Integrins not only transmit signals to cells in response to the extracellular environment (outside-in signaling), but also sense intracellular cues to alter their interaction with extracellular environment (inside-out signaling) (1,2). The aVb5 integrin is expressed in various tissues and cell types, including endothelia, epithelia and fibroblasts (4). It plays a role in matrix adhesion to VN, FN, SPARC and bone sialoprotein (5) and functions in the invasion of gliomas and metastatic carcinoma cells (6,7). aVb5 integrin plays a major role in growth-factor-induced tumor angiogenesis, where cooperative signaling by the aVb5 integrin and growth factors regulates endothelial cell proliferation and survival (8). \n \nApplications:\nSuitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunocytochemistry. Other applications not tested. \n\nRecommended Dilution: \nWestern Blot: 1:1000 \nImmunoprecipitation: 1:50 \nImmunofluorescence (IF-IC): 1:25 \nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-43D

Size

100ul

Applications

ICC, IF, IP, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA. 50% glycerol.

P Type

Pab

Purity

Purified by Protein A and immunoaffinity chromatography.

Isotype

IgG

References

1. Hood, J.D. and Cheresh, D.A. (2002) Nat. Rev. Cancer 2, 91-100. 2. Liu, S. et al. (2000) J. Cell Sci. 113, 3563-3571.\n3. van der Flier, A. and Sonnenberg, A. (2001) Cell Tissue Res. 305, 285-298. 4. Pasqualini, R. et al. (1993) J. Cell Sci. 105, 101-111. 5. Plow, E.F. et al. (2000) J. Biol. Chem. 275, 21785-21788. 6. Sun, L.C. et al. (2007) Cancer Lett. 246, 157-166. 7. Enns, A. et al. (2005) Eur. J. Cancer 41, 1065-1072. 8. Alghisi, G.C. and Ruegg, C. (2006) Endothelium 13, 113-135.

Additional Info

Recognizes endogenous levels of total human integrin b5 protein at ~90kD.