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Integrin, beta8

Cat no: I7661-46

Integrin, beta8

Integrin alpha-V/beta-8 is a receptor for fibronectin.It is a heterodimer of an alpha and a beta subunit. Beta-8 associates with alpha-V and is a single-pass type I membrane protein. It is expressed in placenta, kidney, brain, ovary, uterus and in several transformed cells. Transiently expressed in 293 human embryonic kidney cells. This protein is a member of the integrin beta chain family and encodes a single-pass type I membrane protein with a VWFA domain and four cysteine-rich repeats. This protein noncovalently binds to an alpha subunit to form a heterodimeric integrin complex. In general, integrin complexes mediate cell-cell and cell-extracellular matrix interactions and this complex plays a role in human airway epithelial proliferation. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.\n\nApplications: \nSuitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1:500\nImmunohistochemistry: 1:200\nImmunocytochemistry: 1:200\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1906 IgY, Chicken Pab Rb x Ch \nI1906-10 IgY, Chicken-HRP Rb x Ch \nI1906-12 IgY, Chicken-FITC Rb x Ch \nI1906-14 IgY, Chicken-AP Rb x Ch\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7661-46

Size

50ug

Applications

ICC, IHC, WB

Hosts

Ch/Bird

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgY

References

1. Khan, S. etal., J Biol. Chem. 277: 47826-47833 (2002).

Additional Info

Recognizes human Integrin, beta8.

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