Interferon, gamma, Human, Low Range (IFNg) BioAssay(TM) ELISA Kit

This Human IFN-g ELISA kit is to be used for the in vitro quantitative determination of concentrations in serum, plasma, cell culture supernatant and other biological fluids. It is intended for research use only and is not to be used in diagnostic or therapeutic procedures.\n\nKit Components:\nI7662-16H1: FN-g Microtiter Plate 1x96 wells. Pre-coated with anti-human IFN-g monoclonal antibody.\n\nI7662-16H2: Pab (HRP) 1x14ml. Anti-human IFN-g polyclonal antibody conjugated to horseradish peroxidase with preservative.\n\n*I7662-16H3: IFN-g Standard 2x1vial. Recombinant human IFN-g (3.2ng/vial) in a buffered protein base with preservative, lyophilized.\n \nI7662-16H4: Calibrator Diluent I 1x22ml. Animal serum with preservative. For serum/plasma testing.\n \nI7662-16H5: Calibrator Diluent II (5X) 1x22ml. Cell culture medium with calf serum and preservative. For cell culture supernatant/urine testing.\n \nI7662-16H6: Wash Buffer (20X) 1x60ml, 20-fold concentrated solution of buffered surfactant.\n \nI7662-16H7: Substrate A 1x10ml\nBuffered solution with H2O2.\n\nI7662-16H8: Substrate B 1x10ml\nBuffered solution with TMB.\n \nI7662-16H9: Stop Solution 1x14ml\n2N Sulphuric Acid (H2SO4). Caution: Caustic Material! \n\nSensitivity: \nI7662-16H4: Calibrator Diluent I 13.1pg/ml\nI7662-16H5: Calibrator Diluent II 7.5pg/ml\n\nRange: 0-1600pg/ml\n\nStorage and Stability:\nStore *I7662-16H3 powder at 4 degrees C liquid at -20 degrees C. Store other components at 4 degrees C. Stable for 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n

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SPECIFICATIONS

Catalog Number

I7662-16H

Size

96Tests

Applications

ELISA

References

1. Wheelock,E.F.(1965) Science 149:310 2. Ijzermans, J.M. and R.L Marquet (1989) Immunobiol. 179:456 3. Mogensen, S.C and J.L.Virelizier (1987) Interferon 8:55 4. Grossberg, S.E. et al. (1989) Experientia 45:508 5. Adolf, G.R. (1985) Oncology (Suppl.1)42:33 6. Samuel, C.E. (1991) Virology 183:1 7. Pellegrini, S. and C. Schindler (1993) TIBS 18:338 8. Reiter, Z. (1993) J. Interferon Res. 13:247 9. Farrar, M.A. and R.D. Schreiber (1993) Annu.Rev.Immunol. 11:571 10. Gray, P.W. et al. (1982) Nature 295:503 11. Rinderknecht, E. et al. (1984) J. Biol. Chem.259:6790 12. DeGrado, W.F. et al. (1982) Nature 300:379\n13. Zoon, K.C. et al. (1987) Interferon 9:1 14. Yip, Y.K. et al. (1982) Proc. Natl. Acad. Sci. USA 79:1820 15. Kelker, H.C .et al. (1983) J. Biol. Chem.258:8010 16. Arakawa, T. et al. (1986) J. Interferon Res. 6:687 17. Gray, P.W. and D. Goeddel (1982) Nature 298:859 18. Ealick, S.E. et al. (1991) Science 252:698 19. Lunn, C.A. et al. (1992) J. Biol. Chem.267:17920 20. Paliard, X. et al. (1988) J. Immunol.141:849 21. Christmas, S.E. (1992) Chem. Immunol.53:32 22. Locksley, R.M. and P. Scoff (1991) Immunoparasitology Today A58-A61 23. Billiau, A and R. Dijkmans (1990) Biochem. Pharmacol. 40:1433 24. Bruserud, O. et al. (1993) Eur. J. Hematol. 51:73 25. Sen, G.C. and P. Lengyel (1992) J. Biol. Chem. 267:5017 26. Guessella, G.L.et al. (1993) J. Immunol.151:2725 27. Bulut, V. et al. ((1993) Biochem. Biophys. Res. Commun. 195:1134 28. Espinoza-Delgado, I. (1994) Blood 83:3332 29. Bosco, M.C. et al. (1994) Blood 83:3462 30. ssekutz, A.C. and T.B. Issekutz (1993) J. Immunol. 151:2105 31. Snapper, C.M. et al. (1992) J.Exp.Med.175:1367 32. Snapper, C.M. et al. (1988) J.Immunol. 140: 2121 33 .Halloran, P.F. et al. (1992) J. Immunol.148:3837 34. Thomhill, M.H. et al. (1992) Scand. J. Immunol.38:27

Additional Info

This sandwich ELISA recognizes both natural and recombinant human IFN-g. This kit exhibits no detectable crossreactivity with human; SAA, EGF, IL-6, IL-8, IL-16, M-CSF, GM-CSF, TGF-b1, RANTES, FGF, MIP-1a, CRP, EPO.\n