Interferon gamma (IFNg)

IFN-gamma is a pluripotent cytokine with important pro-inflammatory functions.\n\nApplications:\nCan be used for Neutralization in bio-assays. Furthermore the antibody is useful for Immunohistochemical staining of IFN-gamma containing cells in frozen sections as well as in cells cultured on cover slips and for Western Blot and immunoassay.\n\nRecommended Dilution:\nImmunohistology: 1:10\nWestern Blot: 1:10\nFor neutralization of biological activity dilutions have to be made according to the amounts IFN-gamma to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20 degrees C or colder. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-16K

Size

100ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Rat

Form

Supplied as a liquid in PBS, containing 0.02% sodium azide and 0.1% BSA.

P Type

Pab

Purity

Purified by Protein A affinity chromatography.

Isotype

IgG

Additional Info

Reacts with rat Interferon gamma (IFN-gamma) of both natural and recombinant origin. Inhibits the biological activity of natural and recombinant rat IFN-gamma. Specificity was further assessed by ELISA and Western Blot. No crossreactivities with other cytokines have been detected.