Interferon gamma, Recombinant, Human (IFNg)

Interferon-gamma (IFN-g) is a lymphoid factor which possesses potent anti-viral activity. It has also been shown to stimulate macrophages and NK cells. Human IFN-g is a 16.7kD protein. Interferon gamma is the only known type II interferon. Under physiological conditions, interferon gamma is induced when T-lymphocytes, previously sensitized to an antigen, are exposed to the same antigen, hence its other name: immune interferon. IFNg is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2 , FGF-basic and EGF. Interferon gamma has demonstrated significant anti-proliferative effects in in vivo studies; however, its therapeutic effects have been limited. Endogenous IFN-g has been shown to be required for the protection of mice against mycobacteri and leishmaniasis. In the U.S., human IFN-g has been approved for reducing the frequency and severity of infections in patients with chronic granulomatous disease.\n\nRecombinant Human Interferon gamma produced in E. coli is a single, non-glycosylated, polypeptide chain containing 144 amino acids and having a molecular mass of 16.879kD.\n\nBiological Activity:\nBiological Activity:\nIFNg is fully biologically active when compared to standard. The specific activity as determined in a viral resistance assay is <0.05ng/ml, corresponding to a specific activity of 2.0x10e7 IU/mg. \n\nProtein Content:\nProtein quantitation was carried out by two independent methods:\n1. UV spectroscopy at 280nm using the absorbency value of 0.640 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). \n2. Analysis by RP-HPLC, using a standard solution of IFN-gamma as a Reference Standard.\n\nAmino Acid Sequence:\nMQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-16

Size

100ug

Form

Supplied as a lyophilized powder in 10mM sodium phosphate buffer, pH 7.4. Reconstitute in sterile dH2O, 0.1% HSA or BSA to a concentration (same/more than)0.1mg/ml. Can be further diluted to other aqueous solutions.

Purity

>95% by RP-HPLC and SDS-PAGE. Chromatographically purified.

References

1. Rubinstein, S., Familletti, P.C., Pestka, S.: Convenient Assay for Interferons. J. Virol. 37: 755-758 (1981). 2. Familletti, P. C., Rubinstein, S., Pestka, S.: A Convenient and Rapid Cytopathic Effect Inhibition Assay for Interferon. Methods in Enzymology 78: 387-394 (1981). \n3. Discrimination of Acute Graft-versus-Host Disease from Infections by Enumeration of Peripheral Blood Interferon-gamma Spot-Forming Cells. Transplantation 2006 Feb 27;81(4):632-635\n4. Determination of the Functional Status of Alloreactive T Cells by Interferon-gamma Kinetics. Transplantation 2006 Feb 27;81(4):590-8\n5. Human Allograft Arterial Injury Is Ameliorated by Sirolimus and Cyclosporine and Correlates with Suppression of Interferon-gamma. Transplantation 2006 Feb 27;81(4):559-566\n6. Astragaloside IV Exerts Antiviral Effects Against Coxsackievirus B3 by Upregulating Interferon-gamma. J Cardiovasc Pharmacol 2006 Feb;47(2):190-195\n7. Role of interferon-gamma gene polymorphisms in susceptibility to IgA nephropathy: a family-based association study. Eur J Hum Genet 2006 Feb 22;