Interferon gamma, Recombinant, Mouse (IFNg)

The major interferon produced by mitogenically or antigenically stimulated lymphocytes. It is structurally different from type I interferon and its major activity is immunoregulation. It has been implicated in the expression of class II histocompatibility antigens in cells that do not normally produce them, leading to autoimmune disease. Interferon gamma is produced mainly by T-cells and natural killer cells activated by antigens, mitogens, or alloantigens. It is produced by lymphocytes expressing the surface antigens CD4 and CD8. The synthesis of IFN-gamma is induced, among other things, by Interleukin-2 , FGF-basic , and EGF . \nRecombinant Murine IFN-gamma produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 134 amino acids and having a molecular mass of 15.6kD.\nRecombinant IFN-gamma is purified by proprietary chromatographic techniques. \n\nAmino Acid Sequence:\nMHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC\n\nBiological Activity:\nThe specific activity as determined in a viral resistance assay is <0.1ng/ml, corresponding to a specific activity of 1x10e7U/mg.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, 0.1% HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I7662-17D1

Size

20ug

Applications

ELISA

Form

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute in sterile ddH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml, which can then be further diluted to other aqueous solutions.

Purity

(same/more than)95% as determined by RP-HPLC and SDS-PAGE Silver Stained gel. Chromatographically purified.