Interleukin 1 beta (IL-1b)

Interleukin-1B (IL-1beta) is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. Human IL-1beta is a 17kD protein containing 153 amino acid residues.\n\nApplications: \nSuitable for use in ELISA and Neutralization. Other applications have not been tested.\n\nRecommended Dilutions:\nELISA: Suitable for use as the capture antibody in sandwich ELISA for measuring mouse IL-1b levels using recombinant mouse IL-1b as the standard. Measured IL-1b in mice with a detection range between 10pg/ml-3ng/ml after zymosan induced inflammation.\nNeutralization: Neutralization of the biological activity of IL-1b as demonstrated in an in vivo mouse model. \nOptimal dilution to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. \n

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SPECIFICATIONS

Catalog Number

I7663-11E

Size

100ug

Applications

ELISA

Hosts

Rat

Reactivities

Mouse

Form

Supplied as a liquid in PBS, 0.02% sodium azide.

P Type

Mab

Purity

Purified by Protein G affinity chromatography.

Isotype

IgG1

References

1. Geiger, T., et al.: Neutralization of interleukin-1b activity in vivo with a monoclonal antibody alleviates collagen-induced arthritis in DBA/1 mice and prevents the associated acute phase response. Clin. Exper. Rheumatol. 11: 515-522 (1993). 2. Oluyomi, A.O., et al.: Differential effects of prednisolone and indomethacin on zymosan-induced inflammation in a modified murine tissue-chamber model. Inlamm. Res. 44: 350-356 (1995).

Additional Info

Recognizes recombinant and native mouse Interleukin 1 beta (IL-1b).