Interleukin 1 Receptor AcP, Control Peptide (IL1RacP, IL-1 Receptor Accessory Protein)

Peptide corresponding to aa525-540 of human IL-1RAcP, which is identical to those of mouse and rat origins \nThe pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP) (1). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP (2). IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP (3,4). Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues.\n\nApplications:\nSuitable for use as blocking peptide in Western Blot with I5000-05.\n\nRecommended Dilution:\nBlocking: Incubation of I5000-05P with equal volume of I5000-05 for 30 min. at 37 degrees C typically blocks antibody activity in Western Blot completely.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I5000-05P

Size

50ug

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% BSA, 0.02% sodium azide.

References

1. Greenfeder, S.A., Nunes, P., Kwee, L., Labow, M., Chizzonite, R.A., Ju, G. Molecular cloning and characterization of a second subunit of the interleukin 1 receptor complex. J Biol Chem (1995) 270: 13757-13765. 2. Huang, J., Gao, X., Li, S., Cao, Z. Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein. Proc Natl Acad Sci U S A (1997) 94: 12829-12832. 3. Wesche, H., Korherr, C., Kracht, M., Falk, W., Resch, K., Martin, M.U. The interleukin-1 receptor accessory protein (IL-1RAcP) is essential for IL-1-induced activation of interleukin-1 receptor-associated kinase (IRAK) and stress-activated protein kinases (SAP kinases). J Biol Chem (1997) 272: 7727-7731. 4. Hofmeister, R., Wiegmann, K., Korherr, C., Bernardo, K., Kronke, M., Falk, W. Activation of acid sphingomyelinase by interleukin-1 (IL-1) requires the IL-1 receptor accessory protein. J Biol Chem (1997) 272: 27730-27736.