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Interleukin 10, Human (IL-10), BioAssay(TM) ELISpot Kit

Cat no: I8432-06R

Interleukin 10, Human (IL-10), BioAssay(TM) ELISpot Kit

Intended Use:\nThis 2.5 hour ELISpot kit is developed to detect and visualize of single cells secreting human IL-10.\n\nPrinciple:\nAdd stimulated cells or cells and stimulant to the wells and incubate at 37 degrees C in CO2 incubator for a specified period. Secreted human IL-10 binds to antibody coated ELISpot plate. ELISpot plate coated with human IL-10 Capture Antibody. Cells and unbounded proteins are washed away. Streptavidin-AP or Streptavidin-HRP is added and binds to the biotinylated detection antibody. Unbounded proteins are washed away. Biotinylated detection antibody is added and binds to the secreted human IL-10.\n\nBackground:\nThe mature human IL-10 is a protein with 160 amino acids and the functional IL-10 is a homodimer. IL-10 is expressed by a variety of cells, including mouse T cells (TH1, TH2, and Tr1 subsets), B cells derived from peripheral blood, tonsils or spleen, Epstein

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SPECIFICATIONS

Catalog Number

I8432-06R

Size

96Tests

Applications

ELISA

Reactivities

Hum

References

1. de Waal, M. R., et al. (1991) . Exp. Med. 174:915\n2. de Waal, M. R., et al. (1991) . Exp. Med. 174:1209\n3. de Waal Malefyt, R., Moore, K. M. (1998). The Cytokine Handbook, 3rd edn, (ed, A. Thompson), Interleukin-10 333-346, Academic Press, London\n4. Florentino, D. F., et al. (1991) J. Immunol. 146:3444\n5. Florentino, D. F., et al. (1991) J. Immunol. 147:3815\n6. Grutz G. (2005), J Leukoc Biol. 2005 77,3-15\n7. Moore, K.W., O'Garra, A., et al. (1993). Annu Rev. Immunol. 11, 165-190\n8. Niiro, H., et al. (1994) Int. Immunol. 6:661\n9. Windsor, W. T., et al. (1993). Biochemistry 32, 8807-8815.\n10. Yssel, H., et al. (11991) J. Exp. Med. 174:593\nS7.5(00) IL-10 SL10027E 5

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