Interleukin 12, Recombinant, Human (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)

Interleukin-12 (IL-12) is a potent regulator of cell mediated immune responses. It induces IFN-gamma production by NK and T cells. It is produced by activated monocyte/ macrophage cells, B lymphocytes and connective tissue type mast cells. Among its biological activities, IL-12 promotes the growth and activity of activated NK, CD4+ and CD8+ cells. It induces the development of INF-g producing Th1 cells. Recombinant human IL-12 is a 75kD heterodimeric glycoprotein consisting of disulfide-linked 35kD (p35) and 40kD (p40) subunits. It consists of 503aa.\n\n\nSynonyms:\nNKSF, CTL Maturation Factor (TCMF), Cytotoxic Lymphocyte Maturation Factor (CLMF), TSF.\n\nAA Sequence:\np35- Subunit ---RNLPVAT PDPGMFPCLH HSQNLLRAVS NMLQKARQTL EFYPCTSEEI DHEDITKDKT STVEACLPLE LTKNESCLNS RETSFITNGS CLASRKTSFM MALCLSSIYE DLKMYQVEFK TMNAKLLMDP KRQIFLDQNM LAVIDELMQA LNFNSETVPQ KSSLEEPDFY KTKIKLCILL HAFRIRAVTI DRVMSYLNAS\n\np40 -Subunit- IWELKKDVYV VELDWYPDAP GEMVVLTCDT PEEDGITWTL DQSSEVLGSG KTLTIQVKEF GDAGQYTCHK GGEVLSHSLL LLHKKEDGIW STDILKDQKE PKNKTFLRCE AKNYSGRFTC WWLTTISTDL TFSVKSSRGS SDPQGVTCGA ATLSAERVRG DNKEYEYSVE CQEDSACPAA EESLPIEVMV DAVHKLKYEN YTSSFFIRDI IKPDPPKNLQ LKPLKNSRQV EVSWEYPDTW STPHSYFSLT FCVQVQGKSK REKKDRVFTD KTSATVICRK NASISVRAQD RYYSSSWSEW ASVPCS\n\nStorage and Stablility:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile PBS. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8434-10

Size

2ug

Form

Supplied as a lyophilized powder from PBS pH 7.2.\nReconstitution: Reconstitute with sterile PBS, pH 7.2 to a concentration of 0.2mg/ml.

Purity

Highly purified ((same/more than) 95%, SDS-PAGE and HPLC).\nEndotoxin: (same/less than) 1EU/ug

Additional Info

Biological Activity: The ED50 was determined by the induction of IFN-g from NK cells co-stimulated with IL-18 is (same/less than) 0.1ng/ml, corresponding to a specific activity of (same/more than) 1x10e6u/mg.