Interleukin 12, Recombinant, Rat (IL-12, Cytotoxic Lymphocyte Maturation Factor 1, CTL Maturation Factor, TcMF, CLMF p35, Natural Killer Cell Stimulatory Factor 1, NFSK1, p35, T cell Stimulating Factor, TSF)

Interleukin 12 (IL-12) is a cytokine with important regulatory functions bridging innate and adaptive immunity. It has been proposed as an immune adjuvant for vaccination therapy of infectious diseases and malignancies. The inflammatory properties of IL-12 play an important role in the adjuvant. Interleukin 12 (IL-12) is a proinflammatory cytokine composed by two chains, p40 and p35.It plays a key role in the promotion of a Th1 immune response in the gastrointestinal mucosa. An enhanced expression of IL-12 mRNA in gastric mucosa has been reported in individuals infected by Helicobacter pylori. \n\nRecombinant Rat IL-12 is a glycosylated disulfide linked heterodimeric polypeptide containing 503aa and having a molecular weight of 70kD comprised of disulfide-bonded 35kD (p35) and 40kD (p40) subunits. \n\nAmino Acid Sequence: \nThe sequence of the first five N-terminal amino acids was determined and found to be Arg-Val-Ile-Pro-Val at the p35 subunit and Met-Thr-Glu-Leu-Glu at the p40 subunit.\n\nBiological Activity: \nED50 range=0.01-0.05ng/ml as determined by the dose dependent secretion of interferon-gamma from PHA and IL-2 activated splenocytes that have been costimulated with PMA.\n\nStorage and Stability: \nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8434-17A

Size

2ug

Form

Supplied as a lyophilized powder without additives. Reconstitute with sterile dH2O to >0.1mg/ml. For long term storage it is recommended to add 0.1% HSA or BSA.

Purity

(same/more than)95% (SDS-PAGE).

References

1. Bordetella pertussis inhibition of interleukin-12 (IL-12) p70 in human monocyte-derived dendritic cells blocks IL-12 p35 through adenylate cyclase toxin-dependent cyclic AMP induction. Infect Immun 2006 May;74(5):2831-8. 2. Stat1 deficiency in the host enhances interleukin-12-mediated tumor regression. Cancer Res 2006 Apr 15;66(8):4461-7 3. Interleukin-12 in acute ischemic stroke patients. Folia Neuropathol 2006;44(1):59-66 4. Distinct regulation of CD40-mediated interleukin-6 and interleukin-12 productions via mitogen-activated protein kinase and nuclear factor kappaB-inducing kinase in mature dendritic cells. Immunology 2006 Apr;117(4):526-35 5. Enhanced photocarcinogenesis in interleukin-12-deficient mice. Cancer Res 2006 Mar 15;66(6):2962-9 6. Lactic acid bacteria inducing a weak interleukin-12 and tumor necrosis factor alpha response in human dendritic cells inhibit strongly stimulating lactic acid bacteria but act synergistically with gram-negative bacteria. Clin Vaccine Immunol 2006 Mar;13(3):365-75