Interleukin 13, Human (IL-13) BioAssay(TM) ELISA Kit

This assay employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IL-13 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IL-13 present is bound by the immobilized antibody. After washing away any unbound substances, an enzyme-linked polyclonal antibody specific for IL-13 is added to the wells. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IL-13 bound in the initial step. The color development is stopped and the intensity of the color is measured.\n\nKit Components:\nI8435-01A: Microtiter Plate, 1x96 wells\nI8435-01B: Pab (HRP), 1x21ml\nI8435-01C: Standard*, 1x20ng\nI8435-01D: Assay Diluent: 1x11ml\nI8435-01E: (Cell Culture Supernatant), 1x21ml\nI8435-01F: Calibrator Diluent (Serum/Plasma), 1x21ml\nI8435-01G: Wash Buffer (25X), 1x21ml\nI8435-01H: Color Reagent A (H2O2), 1x12.5ml\nI8435-01J: Color Reagent B (TMB), 1x12.5ml\nI8435-01K: Stop Solution, 1x6ml\n\nStorage and Stability: \nStore all components at 4 degrees C. Stable for 6 months. *Store I8435-01C at -20 degrees C after reconstitution. Components are stable for 1 month at the appropriate temperature after opening/reconstitution.

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SPECIFICATIONS

Catalog Number

I8435-01

Size

1Kit

Applications

ELISA

Reactivities

Hum

References

1. McKenzie, A.N.J. & Zurawski, G., in Guidebook to Cytokines and Their Receptors, Nicola, N.A., ed., Oxford Univ. Press, New York, p. 92 (1994). 2. Zurawski, G. & de Vries, J.E., Immunol. Today 15: 19 (1994). 3. Minty, A., et al., Nature 362: 248 (1993). 4. McKenzie, A.N.J., et al., Proc. Natl. Acad. Sci. USA 90: 3735 (1993). 5. Hilton, D.J., et al., Proc. Natl. Acad. Sci. USA 93: 497 (1996). 6. Lin, J-X., et al., Immunity 2: 331 (1995). 7. Zurawski, S.M., et al., J. Biol. Chem. 270: 13,869 (1995).