Interleukin 16, Recombinant, Human (LCF, prIL-16, FLJ16806, FLJ42735, FLJ44234, HST19289 )

IL-16 is a CD8+ T cell-derived cytokine that induces chemotaxis of CD4+ T cells and CD4+ monocytes and eosinophils. Analysis by gel filtration suggests that, under physiological conditions, hIL-16 exists predominantly as a noncovalently linked multimer. Some IL-16 may exist as a monomer. Only the multimeric form appears to possess chemotactic activity, suggesting that receptor cross-linking may be required for activity. IL-16 also induces expression of IL-2 receptor (IL-2R) and MHC class II molecules on CD4 + T cells. Human and murine IL-16 show significant crossspecies reactivity. Recombinant human IL-16 is a 13.5kD protein consisting of 130 amino acid residues.\n\nBiological Activity: Biological activity is determined by IL-6 abiltiy fo chemoattract CD4+ T-lymphocytes using a concentration range of 1-100ng/ml. For most known in vitro applications, IL-16 exerts it biological activity in the concentration range of 0.1-20ng/ml.\n\nAA Sequence: MPDLNSSTDS AASASAASDV SVESTAEATV CTVTLEKMSA GLGFSLEGGK GSLHGDKPLT INRIFKGAAS EQSETVQPGD EILQLGGTAM QGLTRFEAWN IIKALPDGPV TIVIRRKSLQ SKETTAAGDS\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, carrier protein. Aliquot and store at -20 degrees C. Reconstituted product is stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8438

Size

2ug

Form

Supplied as a lyophilized powder in PBS, pH7.6.\nReconstituted in ddH2O, carrier protein to a concentration of 0.1-1mg/ml.

Purity

Highly purified ((same/more than) 98% by SDS-PAGE analysis). \nEndotoxin: (same/less than) 0.1ng/ug

References

1. Della Bella, S.: Are interleukin-16 and thrombopoietin new tools for the in vitro generation of dendritic cells. Blood 104(13): 4020-8 (12/15/2004). 2. Ferland, C.: IL-16 activates plasminogen-plasmin system and promotes human eosinophil migration into extracellular matrix via CCR3-chemokine-mediated signaling and by modulating CD4 eosinophil expression. Journal of Immunology 173(7): 4417-24 (10/1/2004). 3. Akseli Hemminki: Modulation of coxsackie-adenovirus receptor expression for increased adenoviral transgene expression. Cancer Research 63: 847-853 (2/15/2003). 4. Klimiuk, P.A.: IL-16 as an Anti_Inflammatory Cytokine in Rheumatoid Synovitis1. The Journal of Immunology. 162: 4293-4299 (1/1/1999). 5. Ogasawara, H.: Inhibitory effect of interleukin-16 on interleukin-2 production by CD4+ T cells. Immunology: 96: 215-219 (1/1/1999).