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Interleukin 21, Recombinant, Mouse (IL-21, Interleukin-21, Il21)

Cat no: 149696

Interleukin 21, Recombinant, Mouse (IL-21, Interleukin-21, Il21)

IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. The biological effects of IL-21 include induction of differentiation of T-cells-stimulated-B-cells into plasma cells and memory B-cells, stimulation (in conjunction) with IL-4 of IgG production, and induction of apoptotic effects in naпїЅve B-cells and stimulated B-cells in the absence of T-cell signaling. Additionally, IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the gamma chain (?c) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3. Recombinant mouse IL-21 is a 15kD protein consisting of 130aa.\n\nSource:\nRecombinant protein corresponding to mouse IL-21, expressed in E.coli.\n\nBiological Activity:\nDetermined by a cell proliferation assay using modified Ba/F3 cells. The expected ED50 for this effect is 10-50ng/ml.\n\nEndotoxin:\n<0.1ng/ug (1EU/ug)\n\nAA Sequence:\nMHKSSPQGPD RLLIRLRHLI DIVEQLKIYE NDLDPELLSA PQDVKGHCEH AAFACFQKAK LKPSNPGNNK TFIIDLVAQL RRRLPARRGG KKQKHIAKCP SCDSYEKRTP KEFLERLKWL LQKMIHQHLS\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

149696

Size

2ug

Form

Supplied as a lyophilized powder.

Purity

~98% (SDS-PAGE and HPLC)

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