Interleukin-25, His-Tag (mHis6IL-25, IL-25, IL-17E) (BSA)

IL-25, also known as IL-17E, is a member of the IL-17 superfamily of cytokines. IL-25 is expressed in epithelial cells, CD4+ T cells, mast cells, and eosinophils (1). Many cell types are responsive to IL-25, including T cells, macrophages, and epithelial cells (1). The receptor for IL-25 consists of a heterodimer of IL-17RA and IL-17RB (1,2). IL-25 promotes Th2 type immune responses by induction of IL-5, IL-4, and IL-13 and may contribute to allergic inflammation and asthma (1-3). IL-25 has also been shown in research to promote Th9 cell activation and induce apoptosis in breast cancer cells (4,5).\n\nSource:\nRecombinant protein corresponding to aa17-169 from mouse His6IL-25 expressed in human 293 cells (Accession# NP_542767).\n\nMolecular Weight: \n~20kD\n\nBiological Activity:\nDetermined in mouse splenocyte and HT-29 cell cytokine secretion assays. The ED50 of each lot is between 0.5-1.5ng/ml in mouse splenocyte cells and 0.25-1.5ng/ml in HT-29 cells.\n\nEndotoxin:\n<0.01ng\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

137456

Size

10ug

Form

Supplied as a lyophilized powder in 20mM Tris, pH 7.2, 20ug BSA/ug recombinant IL-25. Reconstitute in sterile 20mM Tris, pH 7.2.

Purity

~97% (SDS-PAGE)

References

1. Iwakura, Y. et al. (2011) Immunity 34, 149-62. 2. Rickel, E.A. et al. (2008) J Immunol 181, 4299-310.\n3. Petersen, B.C. et al. (2012) Nat Med 18, 751-8. 4. Angkasekwinai, P. et al. (2010) Nat Immunol 11, 250-6. 5. Furuta, S. et al. (2011) Sci Transl Med 3, 78ra31.