Interleukin 31 Receptor A, Recombinant, Human (IL-31RA, gp130-Like Monocyte Receptor, GLM-R, GPL)

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100kD type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM-R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are upregulated by interferon- and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 732 amino acid (aa) IL-31 RA contains a 19 aa signal sequence, a 500 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 192 aa cytoplasmic domain. The ECD shares 60%, 58%, 73% and 70% aa identity with mouse, rat, dog and cow IL-31 RA ECD, respectively. Human IL-31 receptors do not respond to mouse IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2). Twelve alternately spliced human IL-31 RA isoforms are known and range in size from 356-745 amino acids. A long (745 aa) and a short (560 aa) transmembrane form are the predominant forms, and many cell lines express both forms (8). The long form, like the 732 aa form, signals by recruiting STAT3, 5 or 1, while the short form does not recruit STATs and inhibits IL-31 signaling. The ratio of these forms and their coexpression with OSM-R determines a cellпїЅs response to IL-31 (8). In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 9, 10).\n\nSource: A DNA sequence encoding the extracellular domain of human IL-31 RA (Ala 20-Ser 516; Accession # Q8NI17) was fused to the signal peptide of human CD-33 at the N-terminus and a 6X histidine tag at the C-terminus. The chimeric protein was expressed in a mouse myeloma cell line, NS0.\n\nDefinition: Measured by its ability to bind rhIL-31 in an ELISA type binding assay with an estimated KD < nM.\n\nApplications: \nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 57.49kD protein migrates as an ~ 95-115kD protein in SDS-PAGE under reducing conditions.\nELISA: type binding assay with an estimated KD < nM.\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile PBS and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8442-41F

Size

50ug

Applications

ELISA, WB

Reactivities

Hum

Form

Supplied as a lyophilized powder in PBS. Reconstitute with sterile PBS to (same/more than) 0.1mg/ml.

Purity

(same/more than) 95%, as determined by SDS-PAGE and visualized by silver stain. Endotoxin (same/less than) 1EU.

References

1. Ghilardi, N. et al., 2002, J. Biol. Chem. 277:16831. 6. Bando, T. et al., 2006, Neuroscience 142:1263.\n2. Diveu, C. et al., 2003, J. Biol. Chem. 278:49850. 7. Broxmeyer, H. E. et al., 2007, Exp. Hematol. 35:78.\n3. Dillon, S. R. et al., 2004, Nat. Immunol. 5:752. 8. Diveu, C. et al., 2004, Eur. Cytokine. Netw. 15:291.\n4. Chattopadhyay, S. et al., 2007, J. Biol. Chem. 282:3014. 9. Bilsborough, J. et al., 2006, J. Allergy Clin. Immunol. 117:418.\n5. Perrigoue, J. G. et al., 2007, J. Exp. Med. 204:481. 10. Sonkoly, E. et al., 2006, J. Allergy Clin. Immunol. 117:411.