Interleukin 32 alpha, Recombinant, Human (IL-32a, Interleukin-32 Precursor, Natural Killer Cells Protein 4, NK4, TAIF, TAIFa, Tumor Necrosis Factor alpha-inducing Factor)

Human IL-32 alpha is one of approximately 6 splice variants of a gene cloned from the human lung carcinoma stable transfectant, A549-Rbeta. IL-32 alpha has been shown to induce IL-8, TNF-a, and MIP-2 production from human & mouse macrophage cell lines. It is upregulated in activated T- & NK-cells, and IFN-g-treated epithelial cells. \n\nBiological Activity: \nHuman IL-32 alpha activity is measured via the dose-dependent induction of TNF-alpha production from human PBMCs. The ED50 is in the range of 0.101-152ng/ml.\n\nAA Sequence:\nMCFPKVLSDD MKKLKARMHQ AIERFYDKMQ NAESGRGQVM SSLAELEDDF KEGYLETVAA YYEEQHPELT PLLEKERDGL RCRGNRSPVP DVEDPATEEP GESFCDKSYG APRGDKEELT PQKCSEPQSS K\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute by adding sterile ddH2O containing 0.1% HSA or BSA. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8442-43

Size

2ug

Form

Supplied as a lyophilized powder from 50mM sodium phosphate, pH 7.5. Reconstitute with sterile ddH2O containing 0.1% HSA or BSA to a concentration of 0.1mg/ml.

Purity

(same/more than) 97% as determined by reducing and non-reducing SDS-PAGE. Endotoxin: (same/less than) 0.1EU/ug