Interleukin 33 ELISA Kit, BioAssay(TM) (IL-33)

For the quantitative determination of Human IL-33 levels in serum or other biological samples.\n*Buffers, Substrates and Plates are not included.\n\nInterleukin-1 (IL-1) family members, such as IL-19 and IL-18, play important roles in host defense, immune regulation, neuronal injury and inflammation (1, 2). Recently, a new member of this family, IL-33, has been reported. It mediates its biological effects via IL-1 receptor ST2 and activates NF-kB and MAPK pathways (3). IL-33 is synthesized in response to cytokines such as IL-1, and plays an important role in TH2-associated immunology. IL-33 expression drives production of TH2-associated cytokines from in vitro polarized TH2 cells and induces the expression of IL-4, IL-5, and IL-13 in vivo. Overexpression of IL-33 in mice leads to severe pathological changes in mucosal organs. IL-33 was previously identified as NF-HEV, a nuclear factor preferentially expressed in high endothelial venules HEV) (4). Recent studies revealed that IL-33 is a heterochromatin-associated nuclear factor and endothelial cells constitute a major source of IL-33 mRNA in chronically inflamed tissues from patients with rheumatoid arthritis and Crohn's disease(5). These data indicate that IL-33 is a dual function protein that may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. The human IL-33 is expressed with a prodomain (aa 1-111) that is cleaved by caspase-1 to produce a 18kD protein (112-270) (6).\n\nKit Contents: \n\nI8443-92Z1: Coating Antibody 1x80ul\nContains affinity-purified Chicken anti-human IL-33 at 1mg/ml.\n\nI8443-92Z2: Calibrator 1x4ul \nContains Recombinant human IL-33 Antigen at 1mg/ml.\nWorking Range: 500

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SPECIFICATIONS

Catalog Number

I8443-92Z

Size

1Kit

References

1. Lee, J. K. et al. Differences in signaling pathways by IL-1beta and IL-18. Proc Natl Acad Sci U S A\n101, 8815-20 (2004).\n2. Allan, S. M., Tyrrell, P. J. & Rothwell, N. J. Interleukin-1 and neuronal injury. Nat Rev Immunol 5,\n629-40 (2005).\n3. Schmitz, J. et al. IL-33, an interleukin-1-like cytokine that signals via the IL-1 receptor-related\nprotein ST2 and induces T helper type 2-associated cytokines. Immunity 23, 479-90 (2005).\n4. Baekkevold, E. S. et al. Molecular characterization of NF-HEV, a nuclear factor preferentially\nexpressed in human high endothelial venules. Am J Pathol 163, 69-79 (2003).\n5. Carriere V, et al. IL-33, the IL-1-like cytokine ligand for ST2 receptor, is a chromatin-associated\nnuclear factor in vivo. Proc Natl Acad Sci U S A. 104, 282-7 (2007).\n6. Martinon, F. & Tschopp, J. Inflammatory caspases: linking an intracellular innate immune system\nto autoinflammatory diseases. Cell 117, 561-74 (2004).