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Interleukin 4, 25-153 aa, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MG

Cat no: I8426-16E

Interleukin 4, 25-153 aa, Recombinant, Human (IL-4, B Cell Stimulatory Factor 1, BSF1, Binetrakin, HCGF, Hodgkin's Cell Growth Factor, IA Inducing Factor, Lymphocyte Stimulatory Factor 1, Macrophage Fusion Factor, Mast Cell Growth Factor 2, MCGF2, MFF, MG

Interleukin-4 (IL-4) is a pleiotropic cytokine that has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of naпїЅve helper T-cell to Th2 cells. Interleukin-4 is a co-stimulator of DNA-synthesis and is induces the expression of class II MHC molecules on resting B-cells. It also enhances both secretion and cell surface expression of IgE and IgG1. Recombinant Interleukin-4 protein, fused to His-tag at N-terminus, was expressed in E. coli and was purified by conventional chromatography techniques, after refolding of the isolated inclusion bodies in a renaturation buffer.\n\nAA Sequence:\nMGSSHHHHHH SSGLVPRGSH MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT \nAQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS\n\nEndotoxin Level: \n(same/less than)1.0 EU per 1 ug of protein (determined by LAL method)\n\nBiological activity: \nThe ED50 for this effect is <1.8ng/ml. Measured in a cell proliferation assay using TF1 human erythroleukemic cells.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8426-16E

Size

50ug

Form

Supplied as a liquid in 20mM Tris-HCl buffer, pH 8.0, 10% glycerol.

Purity

(same/more than) 95% by SDS-PAGE

References

1. Van Kimmenade A., et al. (1988) Eur J Biochem. 173(1):109-14. 2. Yokota T., et al. (1986) Proc. Natl. Acad. Sci. USA. 83(16):5894-8.

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