Interleukin 4, Recombinant, Human (Interleukin-4, IL4, IL-4, BCGF1, BCGF-1, B-cell Stimulatory Factor 1, BSF1, BSF-1, Binetrakin, Lymphocyte Stimulatory Factor 1, MGC79402, Pitrakinra)

Source:\nRecombinant corresponding to 130aa of human Interleukin-4 expressed in E.coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~15kD\n\nBiological Activity:\nThe ED50 as determined by the dose-dependent stimulation of TF-1 cells is less then 0.2ng/ml, corresponding to a Specific Activity of 5x106 IU/mg.\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.594 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8426-02D

Size

10ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. On-column refolding of recombinant human interleukin-4 from inclusion bodies. Protein Expr Purif 2004 Sep;37(1):180-6. 2. Phase I trial of continuous infusion recombinant human interleukin-4 in patients with cancer. Invest New Drugs 2004 Nov;22(4):421-6. 3. Cloning, expression, and purification of soluble human interleukin-4 receptor in Streptomyces. Protein Expr Purif 2004 Jul;36(1):139-45. 4. Optimisation of the CT h4S bioassay for detection of human interleukin-4 secreted by mononuclear cells stimulated by phytohaemaglutinin or by human leukocyte antigen mismatched mixed lymphocyte culture. Immunol Lett 2002 Oct 21;84(1):29-39. 5. Phase II trial of recombinant human interleukin-4 in patients with advanced renal cell carcinoma: a southwest oncology group study. J Immunother 2002 Jul-Aug;25(4):352-8. 6. Detection of spontaneous and antigen-induced human interleukin-4 responses in vitro: comparison of ELISPOT, a novel ELISA and real-time RT-PCR. J Immunol Methods 2002 Feb 1;260(1-2):55-67.