Interleukin 6 Receptor alpha, Human, BioAssay(TM) MAP Kit (CD126, IL6R, IL-6 R alpha)

The multifunctional factor interleukin 6 (IL-6) exerts its activities through binding to a high-affinity receptor complex consisting of two membrane glycoproteins: an 80kD component receptor that binds IL-6 with low affinity (IL-6 R) and a signal-transducing component of 130kD (gp 130) that does not bind IL-6 by itself, but is required for high-affinity binding of IL-6 by the complex. A soluble form of the IL-6 R alpha has been found in the urine of healthy adult humans. This soluble receptor apparently arises from proteolytic cleavage of membrane-bound IL-6 R alpha. No naturally-occurring mRNA encoding a truncated form of the IL-6 R alpha has been reported. Soluble forms of human and murine IL-6 R alphas have been constructed, however, by insertion of termination codons into the regions of the IL-6 R alpha cDNAs encoding the external portions of the receptors and prior to the transmembrane domains. These soluble receptors have been expressed in COS-7 and CHO cells and have been shown to bind to IL-6 in solution and to augment the activity of IL-6 as a result of the binding of the IL-6/IL-6 R alpha complex to membrane-bound gp130.\n\nSample Type:\nCell culture supernates, serum, EDTA plasma, and heparin plasma.\n\nSensitivity:\nThe mean MDD was 1.01pg/ml. The Minimum Detectable Dose (MDD) was determined by adding two standard deviations to the MFI of twenty zero standard replicates and calculating the corresponding concentration. \n\nRange:\n~0.28-3.48pg/ml\n\nSpecificity:\nRecognizes natural and recombinant human IL-6 Ra. \n\nTest Principle:\nHuman IL-6 R alpha BioAssay(TM) Kit afford the user the benefit of multianalyte analysis of biomarkers in a complex sample. For each sample type, a single, multipurpose diluent is used to optimize recovery, linearity, and reproducibility. Such a multipurpose diluent may not optimize any single analyte to the same degree that a unique diluent selected for analysis of that analyte can optimize conditions. Therefore, some performance characteristics may be more variable than those for assays designed specifically for single analyte analysis.\n\nKit Components:\nMicroparticle Concentrate, 100X concentrated, 1x0.075ml\nBiotin-Antibody Concentrate, 100X concentrated, 1x0.075ml\n\nStorage and Stability:\nStore powder at 4 degrees C. Store other components at 4 degrees C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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