Interleukin 8, Recombinant, Human (Interleukin-8, IL8, IL-8, C-X-C Motif Chemokine 8, CXCL8, Emoctakin, Granulocyte Chemotactic Protein 1, GCP1, GCP-1, LECT, LUCT, LYNAP, Monocyte-derived Neutrophil-activating Peptide, MONAP, Monocyte-derived Neutrophil C

Source:\nRecombinant corresponding to 72aa of human Interleukin-8 expressed in E. coli is a single, non-glycosylated polypeptide chain.\n\nMolecular Weight:\n~8.5kD\n\nBiological Activity:\nrHuIL-8 is fully biologically active when compared to standard. Specific Activity of IL-8 in chemotaxis of donor PBL neutrophils, threshold concentration corresponding to 10-100nug/ml\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280nm using the absorbency value of 0.85 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of Eotaxin as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8430-10H

Size

25ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Human immunodeficiency virus-1 Tat protein up-regulates interleukin-6 and interleukin-8 expression in human breast cancer cells. Inflamm Res 2005 Sep;54(9):380-9. 2. Urinary levels of interleukin-6 and interleukin-8 in children with urinary tract infections to age 2. J Urol 2005 Nov;174(5):2011. 3. Comparison of the activities of granulocyte-macrophage colony-stimulating factor and interleukin-8 secretion between two lung epithelial cell lines. J Microbiol Immunol Infect 2005 Oct;38(5):327-31. 4. Measles virus infection induces interleukin-8 release in human pulmonary epithelial cells. Comp Immunol Microbiol Infect Dis 2005 Jul;28(4):311-20. 5. Interleukin-8 regulates expression of Reg protein in Helicobacter pylori-infected gastric mucosa. Am J Gastroenterol 2005 Oct;100(10):2157-66. 6. The -251T allele of the interleukin-8 promoter is associated with increased risk of gastric carcinoma featuring diffuse-type histopathology in Chinese population. Clin Cancer Res 2005 Sep 15;11(18):6431-41.