Inulin is an oligofructofuranoside that is fermented into volatile fatty acids and lactic acid in the large intestine. It is considered a soluble dietary fiber that may lower LDL cholesterol and glucose levels and is a reliable indicator of the glomerular filtration rate in patients with kidney disease. Cayman's Inulin Fluorometric Assay provides a fluorescence-based method for detecting inulin from plasma, serum and urine samples. The Inulin Fluorometric Assay utilizes an enzymatic reaction in which inulin is hydrolyzed into fructose and glucose by inulinase. Fructose is then oxidized to 5-keto-D-Fructose by D-Fructose Dehydrogenase, with the concomitant reduction of the fluorescent substrate to yield a highly fluorescent product. The fluorescent product is analyzed with an excitation wavelength of 530-540 nm and an emission wavelength of 585-595 nm.