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IP-10 (Biotin)

Cat no: I8449A

IP-10 (Biotin)

Human IP10 is an 8.6kD protein produced by several cell types during the delayed type hypersensitivity response. IP10 acts as a chemoattractant towards monocytes, lymphocytes and certain T cells.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA (Direct): 0.5ug/ml (100ul/well ) will detect 0.2-0.4ng/well of recombinant hIP-10.\nWestern Blot: 0.1-0.2ug/ml. Detects 1.5-3.0ng/lane recombinant hIP-10 under either reducing or non-reducing conditions.\nOptimal dilutions to be determined by researcher.\n\nStorage and Stability:\nLyophilized powder may be stored at 4 degrees C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20 degrees C. Reconstituted product is stable for 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

I8449A

Size

25ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Conjugates

Biotin

Form

Supplied as a lyophilized powder from PBS pH 7.4. No preservatives added. Labeled with Biotin. Reconstsitute with sterile PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

Additional Info

Recognizes IP10.

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