JNK1 alpha1, unactive Recombinant Human (c-Jun N-terminal Kinase 1, JNK, JNK-46, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, MAPK8, PRKM8, Stress-activated Protein Kinase 1c, SAPK1c, SAPK1, Stress-activated Protein Kinase JNK1)

Jun Kinases (JNK), also called stress-activated protein (SAP) kinases, are proline-directed, serine/threonine- specific kinases that require phosphorylation on tyrosine for phosphotransferase activity. JNK was originally shown to phosphorylate microtubule-associated protein-2, but c-Jun and ATF2 appears to be an important physiological target of this kinase. Phosphorylation of Thr69 and Thr71 of ATF2 by JNK is important for the transcriptional activity of ATF2. cDNA cloning studies with rat and human libraries have shown that there are several isoforms of the JNK family. Many stimuli activate JNK including heat shock, hyperosmotic shock, UV irradiation, exposure to protein synthesis inhibitors such as cycloheximide and anisomycin, and treatment with proinflammatory cytokines such as tumor necrosis factor-a.\n\nSource: \nRecombinant full-length human JNK1a1/SAPK1c with an N-terminal His-tag, expressed in Sf21 cells. \n\nAccession Number: \nL26318\n\nSpecific Activity: \n< 1% of maximum activity. Activated by phosphorylation with MKK4 and MKK7beta1.\n\nJNK1a1/SAPK1c Kinase Assay:\n2uM of unactive JNK1a1/SAPK1c was activated using 0.1uM MKK4 and 0.1uM MKK7b1.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

J2500-05

Size

50ug

Form

Supplied as a liquid in 50ul of 50mM Tris-HCl, pH 7.5, 150mM sodium chloride, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.02% Brij-35, 50% glycerol.

Purity

Purified using Ni-NTA agarose. Purity (same/more than)90%. Mr ~45kD. Purity was assessed by SDS-PAGE and Coomassie blue staining of 3ug of unactive JNK1a1.

References

1. Gupta, S., et al., EMBO J. 15: 2760

Alternative Names

EC=2.7.11.24