KCC3a (K, Cl Cotransporter 3a)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.\n\nThe K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes. This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia. The net direction of this transport is out of the cells. At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized. KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini.\n\nKCC3 is ~77% identical to KCC1. Alternative splicing of KCC3 transcripts generates KCC3 with different N-termini (1099 aa and 1150 aa). It is highly expressed in brain, heart, skeletal muscle, and kidney. KCC3 is modulated by VEGF and TNF-alpha.\n\nApplications: \nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence technique.\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: K0131-05\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0131-06

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.4, 0.1% BSA, 0.05% sodium azide, 40% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

References 1. Hiki K et al (1999) J Biol. Chem 274, 10661-10667; Mount DB et al (1999) 274, 16355-16362; Mount DB et al (1998) J Exptl. Biol. 201, 2091; O'Neill WC (1999) Am J. Physiol. 276, C995.

Additional Info

Recognizes human KCC3a. Ssequence homology: both alternatively spliced forms of the human KCC3 (1099 aa) and KCC3a (1150 aa). Species sequence homology: mouse and monkey 100%; frog. 82%.