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KCC4 (K, Cl Cotransporter 4)

Cat no: K0135-01

KCC4 (K, Cl Cotransporter 4)

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane. \nThe K-Cl cotransporters mediate the coupled movement of K+ and Cl- ions across the cell membranes. This transport process is involved in the regulatory volume decrease in response to cell swelling in red cells and vectorial movement of Cl- ions across the kidney epithelia. The net direction of this transport is out of the cells. At least four isoforms of KCC identified (KCC1, KCC2, KCC3, and KCC4) have been identified and functionally characterized. KCC are predicted to have the same protein structure: 12 transmembrane domains with a large extracellular loop with potential N-glycosylation sites, and the cytoplasmic N and C-termini. Most recently, KCC4 (mouse and human 1083 aa) have been cloned that are 65-71% identical to KCC1/KCC2. It is highly expressed in heart and kidney.\n\nApplications:\nSuitable for use in Western Blot and ELISA. Other applications not tested.\n\nRecommended Dilution:\nWestern Blot: 1-10ug/ml using Chemiluminescence technique.\nELISA: 1:10,000-1:100,000; using 50-100ng of control peptide/well (K0135-01B).\nOptimal dilutions to be determined by the researcher.\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 12 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0135-01

Size

50ug

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

Hum

Form

Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide, before the addition of glycerol to 40%.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Hiki K et al (1999) J Biol. Chem 274:10661-10667. 2. Mount DB et al (1999) 274:16355-16362. 3. Mount DB (1998) J Exptl. Biol. 201:2091.

Additional Info

Recognizes human KCC4. Species Sequence Homology: mouse (89%). No significant sequence homology was found with other KCCs or CCC.

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