Keratin-18, ID (Keratin, Type I Cytoskeletal 18, Cytokeratin-18, CK-18, Dorsal Aorta Proneprin Kinesin-1, DreK18, Keratin-18, K18, dapk1, krt18)

Intermediate filaments (IFs), together with actin microfilaments and tubulin microtubules, comprise the three major cytoskeletal networks that are found in most eukaryotic cells. Keratins, which are the largest IF protein subgroup, can be divided into the acidic type I (K9-K20) and basic type II (K1-K8) keratins. Typically, epithelial cells express at least one of each type, and the proteins exist in the cells as noncovalent obligate heteropolymers. Keratin networks are highly dynamic and reorganized during cell differentiation, mitosis and apoptosis, and also play important cytoprotective and structural support roles for the cell. During apoptosis, the epithelial keratins 18 and 19 undergo caspase- mediated proteolysis, leading to the disassembly of the nuclear envelope and cytoplasmic IF networks.\n\nApplications:\nSuitable for use in ELISA and Western Blot. Other applications not tested.\n\nRecommended Dilution:\nELISA: 1:5000-1:20,000\nWestern Blot: 1:500-1:1000\nOptimal dilutions to be determined by the researcher.\n\nControl Peptide: K0199-22Y\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Prices direct from United States Biological

Quick response times

Exclusive Biosave savings/discounts

SPECIFICATIONS

Catalog Number

K0199-22V

Size

250ul

Applications

ELISA, WB

Hosts

Rabbit

Reactivities

ZF/Fish

Form

Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Ku NO et al. Am J. Physiol. 277:G1108-37 (1999). 2. Schweizer J et al. J. Cell Biol. 174(2):169-74 (2006). 3. Omary MB et al. et al. Trends Biochem. Sci. 31:383-394 (2006). 4. Omary MB et al. N. Engl. J. Med. 351:2087-2100 (2004) 5. Ku NO et al. J. Biol. Chem. 272: 33197-33203 (1997).

Additional Info

Recognizes zebrafish Keratin-18.