Keratin, HMW (High Molecular Weight Keratin)

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50пїЅ, 48, 46, 45, and 40kD. The basic keratins have molecular weights of 65-67, 64, 59, 58, 56 and 52kD. Members of acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with cell type, differentiation status and environment. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.\n\nApplications: \nSuitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Other applications not tested.\n\nRecommended Dilutions:\nImmunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining of formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, 10 minuts at 37 degrees C or Protease XXV at 1mg/ml PBS for 5 minutes at 37 degrees C.\nOptimal dilutions to be determined by the researcher.\n\nPositive Control:\nSkin. Lung carcinoma\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K0199-75

Size

500ul

Applications

ELISA, IF, IHC

Hosts

Mouse

Reactivities

Hum, Mouse, Rat, Bov, Ch/Bird, NHP, Rabbit

Form

Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA. See K0199-75X

P Type

Mab

Purity

Purified

Isotype

IgG1

References

1. Woodcock-Mitchell J; Eichner R; Nelson WG; Sun TT. Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. Journal of Cell Biology, 1982 Nov, 95(2 Pt 1):580-8. 2. Tseng SC; Jarvinen MJ; Nelson WG; Huang JW; Woodcock-Mitchell J; Sun TT. Correlation of specific keratins with different types of epithelial differentiation: monoclonal antibody studies. Cell, 1982 Sep, 30(2):361-72. 3. Eichner R; Bonitz P; Sun TT. Classification of epidermal keratins according to their immunoreactivity, isoelectric point, and mode of expression. Journal of Cell Biology, 1984 Apr, 98(4):1388-96. 4. Cooper D; Schermer A; Pruss R; Sun TT. The use of aIF, AE1, and AE3 monoclonal antibodies for the identification and classification of mammalian epithelial keratins. Differentiation, 1984, 28(1):30-5.

Additional Info

Recognizes the 65-67, 64, 59, 58, 56 and 52kD human keratins of the basic subfamily. Species Crossreactivity: monkey, bovine, rabbit, mouse, rat and chicken.