Keratinocyte Growth Factor, Recombinant, Human (KGF, Fibroblast Growth Factor 7, FGF7, FGF-7, Heparin-binding Growth Factor 7, HBGF-7)

Source:\nRecombinant corresponding to 164aa of human Keratinocyte Growth Factor expressed in E.coli is a single, non-glycosylated, polypeptide chain.\n\nMolecular Weight:\n~19kD\n\nBiological Activity:\nThe ED50, calculated by the dose-dependent stimulation of KGF-responsive BaF3 indicator cells (measured by 3H-thymidine uptake).\n\nDimers and Aggregates:\n(same/less than)1% (SDS-PAGE)\n\nProtein Content:\nProtein quantitation was carried out by two independent methods:1. UV spectroscopy at 280nm using the absorbency value of 0.9 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of KGF as a Reference Standard.\n\nStorage and Stability:\nLyophilized powder may be stored at -20 degrees C. Stable for 12 months at -20 degrees C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K0201-15E

Size

2ug

Form

Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to (same/more than)0.1mg/ml.

Purity

~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: (same/less than)0.1ng/ug (IEU/ug)

References

1. Keratinocyte Growth Factor (KGF) Is Required For Post-Natal Thymic Regeneration. Blood 2005 Nov 22. 2. KGF induces lipogenic genes through a PI3K and JNK/SREBP-1 pathway in H292 cells. J Lipid Res 2005 Dec;46(12):2624-35. 3. KGF prevents oxygen-mediated damage in ARPE-19 cells. Invest Ophthalmol Vis Sci 2005 Sep;46(9):3435-42. 4. Stimulation of human hair growth by the recombinant human keratinocyte growth factor-2 (KGF-2). Biotechnol Lett 2005 Jun;27(11):749-52. 5. KGF-induced motility of breast cancer cells is dependent on Grb2 and Erk1,2. Clin Exp Metastasis 2004;21(5):437-43. 6. Comparative effects of glucagon-like peptide-2 (GLP-2), growth hormone (GH), and keratinocyte growth factor (KGF) on markers of gut adaptation after massive small bowel resection in rats. JPEN J Parenter Enteral Nutr 2004 Nov-Dec;28(6):399-409.