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KIR2DL1, Recombinant, Human (p58 KIR, p58.1, CD158, cl-42, KIR-K6, NKAT1, NKR-K6, Killer Cell Immunoglobulin (Ig)-like Receptor)

Cat no: K1858-40

KIR2DL1, Recombinant, Human (p58 KIR, p58.1, CD158, cl-42, KIR-K6, NKAT1, NKR-K6, Killer Cell Immunoglobulin (Ig)-like Receptor)

An inhibitory Killer Cell Ig-like Receptor (KIR, previously called p58 KIR, p58.1, cl-42, NKAT1, or KIR-K6), which recognizes class I MHC molecules (HLA-Cw2, -Cw4, -Cw5, and Cw6). The protein coding region of the extracellular domain of KIR2DL1 (amino acids 1-202) was\ncloned into an E. coli expression vector. The extracellular domain of KIR2DL1 was overexpressed as insoluble protein aggregates(inclusion bodies).\n\nMolecular Weight: \n22.2kD\n\nSequence: \nMEGVHRKPSL/ LAHPGRLVKS/ EETVILQCWS/ DVMFEHFLLH/ REGMFNDTLR/ LIGEHHDGVS/\nKANFSISRMT/ QDLAGTYRCY/ GSVTHSPYQV/ SAPSDPLDIV/ IIGLYEKPSL/ SAQLGPTVLA/ GENVTLSCSS/ RSSYDMYHLS/ REGEAHERRL/ PAGPKVNGTF/ QADFPLGPAT/ HGGTYRCFGS/\nFHDSPYEWSK/ SSDPLLVSVT/ GN\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. For long-term storage, store at -20 degrees C. Aliquots are stable for at least 6 months at -20 degrees C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

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SPECIFICATIONS

Catalog Number

K1858-40

Size

100ug

Form

Supplied as a liquid in 20mM Tris-HCl, pH 7.5.

Purity

(same/more than) 95% by SDS PAGE; Purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a redox buffer.

References

1. Steffens, U., et al., Tissue Antigens 51: 398-413 (1998). 2. Wagtmann, N.R., et al., Immunity 2: 439-449 (1995). 3. Colonna, M., et al., Science 269: 405-408 (1995). 4. Kim, J., et al., J. Immunol. 159: 3875-3882 (1997).

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