Klotho (KL)

Animal models of human aging which display the characteristic diseases associated with aging provide insight into the cause of such diseases. Recently a transgenic mouse model, called KLOTHO, with several premature aging phenotypes has been described. They display premature aging phenotypes such as Osteoporosis, age related skin changes, ectopic calcifications, atrophy of genital organs and thymus, emphysema and short life span. KL protein associated with the KLOTHO mutation is a 1014 amino acid long peptide. It has a putative signal sequence at its N-terminus and a single transmembrane domain near its C-terminus, which is postulated to anchor it to the membrane. kl gene expression was observed to be tissue specific. Improvement of systemic aging phenotypes in kl/kl mice occurs even when the exogenous expression was limited to some organs, suggesting that KL associated aging is regulated through a humoral signaling pathway. KL has been reported to be localized on the cell surface when expressed on CHO cells. Human kl cDNA is expected to encode a protein of 1012 amino acids and has 86% homology to mouse kl.\n\nApplications: \nSuitable for use in ELISA, Immunohistochemistry and Western Blot. Other applications not tested.\n\nRecommended Dilutions:\nELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well.\nWestern Blot: 1-10ug/ml using ECL.\nImmunohistochemistry: 2-20ug/ml\nOptimal dilutions to be determined by the researcher.\n\nRecommended Control Peptide:\nK1893-03: KLOTHO, Mouse, Control Peptide (KL)\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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SPECIFICATIONS

Catalog Number

K1893-02

Size

50ug

Applications

ELISA, IHC, WB

Hosts

Rabbit

Reactivities

Mouse

Form

Supplied as liquid in PBS, 0.1% BSA.

P Type

Pab

Purity

Purified by immunoaffinity chromatography.

Isotype

IgG

References

1. Segawa H (2006) Am J Physiol Renal Physiol, Feb 2007; 292:F769-F779. 2. Kurosu H (2006) JBC 281:6120-6123. 3. Koro-o, M., et al., (1997) Nature 390:45-51. 4. Martin, G.M. & Mian, I.S., (1997) Nature 390:18-19.

Additional Info

Recognizes mouse KLOTHO (KL). No significant homology with other known proteins.