LacI

The lac repressor protein (Lac I) is an important genetic control protein. It controls the expression of the lactose metabolizing enzymes in Escherichia coli. This regulation involves specific recognition of the operator DNA sequence (Lac O) and consequent inhibition of transcription initiation by RNA polymerase; modulation of binding at this target site by small sugar molecules, inducers, provides the basis for regulation. Different ligand binding activities are associated with the isolated domains of Lac I: specific and nonspecific DNA binding with the NH2 termini, and inducer and specific DNA binding with the core protein.\n\nApplications: \nSuitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications have not been tested.\n\nRecommended Dilutions: \nWestern Blot: 0.5-2ug/ml. Detects LacI from LacI transfected Cos-1 whole cell lysate.\nImmunocytochemistry: Reported to show nuclear immunostaining in LacI transfected cells fixed with methanol.\nImmunohistochemistry: Reported to show nuclear immunostaining in LacI transgenic tissue fixed with 4% paraformaldehyde and permeabilized with Triton X-100.\nOptimal dilutions to be determined by the researcher.\n\nRecommended Secondary Antibodies:\nI1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\nI1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt x Mo\n\nStorage and Stability:\nMay be stored at 4 degrees C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degrees C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

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